IFN alpha 2b Protein (Arg46)
This Recombinant IFN alpha 2b protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN1096852
发货至:
中国
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Quick Overview for IFN alpha 2b Protein (Arg46) (ABIN1096852)
抗原
IFN alpha 2b
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Functional Studies (Func)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- Arg46
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原理
- Recombinant Human Interferon α2B Variant (Arg46)/IFN-α2B
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序列
- MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
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产品特性
- Recombinant Human Interferon-alpha2b produced in E. coli is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19,4 kDa. The Interferon-alpha2b gene was obtained from human leukocytes.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Biological Activity Comment
- Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV).
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IFN alpha 2b (AA 24-188) (Active) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- IFN alpha 2b
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别名
- IFN-alpha 2B
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背景
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At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.
Alternative Names: Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2 -
分子量
- 19.4 kDa
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UniProt
- P01563
抗原
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