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G-CSF Protein (AA 31-204)

This Recombinant G-CSF protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN1096696
发货至: 中国
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Quick Overview for G-CSF Protein (AA 31-204) (ABIN1096696)

抗原

See all G-CSF (CSF3) 蛋白
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

蛋白类型

Recombinant

生物活性

Active

宿主

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大肠杆菌(E. Coli)

应用范围

Functional Studies (Func)

纯度

> 95 % as determined by reducing SDS-PAGE.
  • 产品特性

    AA 31-204

    原理

    Recombinant Human Granulocyte Colony-Stimulating Factor/G-CSF

    序列

    MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

    产品特性

    Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.

    过滤

    0.2 μm filtered

    内毒素水平

    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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  • 说明

    Biological activity: ED50 is less than 0.1 ng/ml as calculated by the dose-dependent proliferation of murine NFS-60 indicator cells.Specific Activity of 6.0 x 107 IU/ mg.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    缓冲液

    Lyophilized from a 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004 % Tween 80, 5 % Mannitol, pH 4.0.

    储存条件

    4 °C,-20 °C

    储存方法

    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    有效期

    3 weeks
  • 抗原

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    别名

    g-csf

    背景

    Alternative Names: Granulocyte Colony-Stimulating Factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSFRecombinant Human G-CSF: Granulocyte-Colony-Stimulating Factor is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
    Alternative Names: Recombinant Human G-CSF, Granulocyte Colony-Stimulating Factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF

    分子量

    18.8 kDa

    UniProt

    P09919-2

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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