PrxQ 抗体
PRXQ
适用: Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays
WB
宿主: 兔
Polyclonal
unconjugated
(3 references)-
- 抗原
- PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
- 适用
- Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- 预测反应
- Populus spp. , monocots including Triticum aestivum, Oryza sativa
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 16 kDA
- 纯化方法
- serum
- 免疫原
- His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
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- 应用备注
- 1: 5000 with standard ECL (WB)
- 说明
-
In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 200 μL of sterile water.
- 注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).
: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." in: Journal of experimental botany, (2010) (PubMed).
: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).
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: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).
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- 抗原
- PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
- 别名
- PrxQ
- 背景
-
AGI Code: At3g26060
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence. - 分子量
- 16 kDA
- UniProt
- Q9LU86
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