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PrxQ 抗体

This anti-PrxQ antibody is a 兔 多克隆 antibody detecting PrxQ in WB. Suitable for Arabidopsis thaliana, Manihot esculenta, Spinach 和 Zea mays. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN125852
发货至: 中国

Quick Overview for PrxQ 抗体 (ABIN125852)

抗原

PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))

适用

Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays

宿主

克隆类型

多克隆

应用范围

Western Blotting (WB)
  • 交叉反应 (详细)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    预测反应

    Populus spp. , monocots including Triticum aestivum, Oryza sativa

    产品特性

    Expected / apparent Molecular Weight of the Antigene: 16 kDA

    纯化方法

    serum

    免疫原

    His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
  • 应用备注

    1: 5000 with standard ECL (WB)

    说明

    In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 200 μL of sterile water.

    注意事项

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Wu, Han, Wang, Wang, Sun, Zu, Chen: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).

    Pulido, Spuinola, Kirchsteiger, Guinea, Pascual, Sahrawy, Sandalio, Dietz, Gonzualez, Cejudo: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." in: Journal of experimental botany, (2010) (PubMed).

    Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).

  • 抗原

    PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))

    别名

    PrxQ

    背景

    AGI Code: At3g26060
    Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.

    分子量

    16 kDA

    UniProt

    Q9LU86
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