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Prolyl Endopeptidase FAP Protein (FAP) (His tag)

This Recombinant Prolyl Endopeptidase FAP protein is expressed in HEK-293 Cells.
产品编号 ABIN7197521
发货至: 中国

Quick Overview for Prolyl Endopeptidase FAP Protein (FAP) (His tag) (ABIN7197521)

抗原

Prolyl Endopeptidase FAP (FAP)

蛋白类型

Recombinant

生物活性

Active

宿主

资源

HEK-293 Cells

纯度

> 95 % as determined by reducing SDS-PAGE.
  • 标记

    This Prolyl Endopeptidase FAP protein is labelled with His tag.

    原理

    Recombinant Human FAP/Seprase Protein (His Tag)(Active)

    序列

    Leu 26-Asp 760

    产品特性

    A DNA sequence encoding the human FAP isoform 1 (Q12884-1) extracellular domain (Leu 26-Asp 760) was fused with the polyhistidie-tag at the N-terminus.

    内毒素水平

    < 1.0 EU per μg as determined by the LAL method.

    Biological Activity Comment

    Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1200 pmol/min/μg
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Please refer to the printed manual for detailed information.

    缓冲液

    Lyophilized from sterile 25 mM Tris, 250 mM NaCl, pH 8.2

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • 抗原

    Prolyl Endopeptidase FAP (FAP)

    别名

    FAP/Seprase

    背景

    Background: Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    Synonym: DPPIV;DPPIVA;FAPA;Fibroblast Activation Protein alpha;SIMP

    分子量

    87.2 kDa
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