Potassium Channel KAT3 抗体
Our Local Distributor
北京 101111
Quick Overview for Potassium Channel KAT3 抗体 (ABIN488529)
抗原
适用
宿主
克隆类型
应用范围
-
-
原理
- Anti-KC1 | potassium channel KAT3
-
交叉反应 （详细）
-
Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Arabidopsis thaliana
-
纯化方法
- Immunogen affinity purified serum
-
免疫原
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960
-
-
-
应用备注
- 1 : 50 with 125I (WB)
-
说明
-
In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- For reconstitution add 100 μL of sterile water
-
缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
-
注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
-
储存条件
- -20 °C
-
储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
-
-
- Potassium Channel KAT3 (KAT3)
-
别名
- KC1
-
背景
-
Background: KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Additional information: For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
-
分子量
- 75.5 | 75 kDa
-
基因ID
- 829400
-
UniProt
- P92960
抗原
-
