Potassium Channel KAT3 抗体
This anti-Potassium Channel KAT3 antibody is a 兔 多克隆 antibody detecting Potassium Channel KAT3 in WB. Suitable for Arabidopsis thaliana.
Quick Overview for Potassium Channel KAT3 抗体 (ABIN488529)
抗原
Potassium Channel KAT3 (KAT3)
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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预测反应
- Arabidopsis thaliana
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 75.5 / 75 kDa
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纯化方法
- serum
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免疫原
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
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应用备注
- 1: 50 with 125I (WB)
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说明
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In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water.
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注意事项
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Potassium Channel KAT3 (KAT3)
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别名
- KC1
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背景
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AGI Code: At4g32650
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC -
分子量
- expected: 75.5 kDa, apparent: 75 kDa
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UniProt
- P92960
抗原
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