Nuclear Prelamin A Recognition Factor (NARF) (Middle Region) Peptide
NARF
适用: 人
宿主: 合成
BP, WB
Quick Overview for Nuclear Prelamin A Recognition Factor (NARF) (Middle Region) Peptide (ABIN980494)
抗原
NARF
(Nuclear Prelamin A Recognition Factor (NARF))
宿主
人
资源
合成
应用范围
Blocking Peptide (BP), Western Blotting (WB)
-
-
蛋白结构域
- Middle Region
-
产品特性
- This is a synthetic peptide designed for use in combination with anti-NARF antibody (Catalog #: ARP54490_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
-
纯化方法
- Purified
-
-
-
-
应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
-
浓度
- 1 mg/mL
-
缓冲液
- Final peptide concentration is 1 mg/mL in PBS.
-
注意事项
- Avoid repeated freeze-thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
-
-
- NARF (Nuclear Prelamin A Recognition Factor (NARF))
-
背景
-
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Alias Symbols: DKFZp434G0420, FLJ10067, IOP2
Protein Interaction Partner: LMNA,LMNA
Protein Size: 502 -
分子量
- 55 kDa
-
基因ID
- 26502
-
NCBI登录号
- NM_031968, NP_114174
抗原
-
You are here:
