V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Peptide
AKT1
适用: 人
BP
Sodium azide
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- 抗原 See all AKT1 products
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 蛋白类型
- Recombinant
- 宿主
- 人
- 应用范围
- Blocking Peptide (BP)
- 产品特性
- Concentration Definition: by dry weight
- 过滤
- Sterile filtered
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- 应用备注
- Akt blocking peptide should be used at 1.0 µg per 1.0 µl of antiserum in per assay.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 别名
- AKT Peptide, CWS6 Peptide, PKB Peptide, PKB-ALPHA Peptide, PRKBA Peptide, RAC Peptide, RAC-ALPHA Peptide, Akt Peptide, Rac Peptide, PKB/Akt Peptide, PKBalpha Peptide, AKT/PKB Peptide, AKT1 Peptide, Akt/PKB Peptide, Akt1 Peptide, CG4006 Peptide, D-Akt Peptide, DAKT1 Peptide, DAKT1/PKB Peptide, DAkt Peptide, DAkt1 Peptide, DPKB Peptide, DRAC-PK Peptide, DRAC-PK66 Peptide, DRAC-PK85 Peptide, Dakt Peptide, Dakt1 Peptide, Dmel\\CG4006 Peptide, Dpkb Peptide, PKB/AKT Peptide, PKB/dAKT Peptide, RacPK Peptide, akt Peptide, akt1 Peptide, dAKT Peptide, dAKT/dPKB Peptide, dAKT1 Peptide, dAkt Peptide, dAkt/PKB Peptide, dAkt1 Peptide, dPKB Peptide, dakt Peptide, dakt1 Peptide, l(3)04226 Peptide, l(3)89Bq Peptide, p-Akt Peptide, pAkt Peptide, ACT-5 Peptide, akt-1 Peptide, pkb Peptide, v-akt Peptide, v-akt1 Peptide, xAct Peptide, ATAKT1 Peptide, F18A8.2 Peptide, F18A8_2 Peptide, K+ transporter 1 Peptide, POTASSIUM TRANSPORTER Peptide, AKT serine/threonine kinase 1 Peptide, thymoma viral proto-oncogene 1 Peptide, CG4006 gene product from transcript CG4006-RE Peptide, actin beta Peptide, v-akt murine thymoma viral oncogene homolog 1 S homeolog Peptide, v-akt murine thymoma viral oncogene homolog 1 Peptide, K+ transporter 1 Peptide, Serine/threonine-protein kinase akt-1 Peptide, AKT1 Peptide, Akt1 Peptide, ACTB Peptide, akt1.S Peptide, akt1 Peptide, KT1 Peptide, akt-1 Peptide
- 背景
- Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodies, but can also occur with monoclonals as well. To determine which band or staining is specific, the AKT immunizing peptide blocking experiment can be performed. Prior to the staining protocol, the anti-AKT antibody is neutralized (incubated with an excess of peptide that corresponds to the epitope recognized by the antibody). The AKT antibody that is bound to the blocking peptide is no longer available to bind to the epitope present in the protein on the Western blot or in the cell. The neutralized antibody is then used side-by-side with the antibody alone, and the results are compared. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is specific: this staining will be absent from the Western blot or immunostaining performed with the neutralized antibody.
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