Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) Peptide
POLR3A
适用: 哺乳动物
宿主: 合成
BP, WB, IHC
Quick Overview for Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) Peptide (ABIN937013)
抗原
POLR3A
(Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
宿主
哺乳动物
资源
合成
应用范围
Blocking Peptide (BP), Western Blotting (WB), Immunohistochemistry (IHC)
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蛋白类型
- Synthetic
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序列
- AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT
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产品特性
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A synthetic peptide for use as a blocking control in assays to test for specificity of POLR3 A antibody,
Alternative Names: POLR3A control peptide, POLR3A antibody Blocking Peptide, Anti-POLR3A Blocking Peptide, RNA Polymerase IIIA Blocking Peptide, RPC1 Blocking Peptide, RPC155 Blocking Peptide, hRPC155 Blocking Peptide, POLR3A, POLRA-3, POLRA 3, POLRA-3 Blocking Peptide, POLRA 3 Blocking Peptide
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应用备注
- Optimal conditions should be determined by the investigator
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 100 µL of distilled water for a final peptide concentration is 1 mg/mL.
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缓冲液
- PBS
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C long term.
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- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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背景
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
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分子量
- 156 kDa
抗原
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