Purified recombinant E.coli DsbG protein Expression System: E.coli Molecular weight on SDS-PAGE will appear higher.
纯度
> 95 % pure
应用备注
DsbG protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM EDTA, and 10 % glycerol.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
储存方法
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
抗原
DsbG
背景
Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25 % the level of DsbC. Recombinant DsbG was expressed in E. coli and purified by conventional chromatography techniques. Alternative Names: ybdP protein, Thiol:disulfide interchange protein dsbG ECK0598 protein, Periplasmic disulfide isomerase/thiol disulphide oxidase protein, , JW0597 protein