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- 抗原
- DnaK
- 蛋白类型
- Recombinant
- 蛋白结构域
- AA 385-546
- 宿主
- 所有种类
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST
- 产品特性
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Purified recombinant E.coli Dnak protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
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DnaK (AA 385-638), (C-Term) peptide
适用: 所有种类 宿主: 大肠杆菌（E. Coli） SDS Dithiothreitol (DTT)
DnaK (AA 508-638) peptide适用: 所有种类 宿主: 大肠杆菌（E. Coli） SDS Dithiothreitol (DTT)
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- 应用备注
- Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 2 mM beta-mercaptoethanol, and 1 mM EDTA.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- DnaK
- 背景
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DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Alternative Names: Heat shock protein 70 protein, Chaperone Hsp70 protein, dnaK protein, seg protein, grpF protein, Heat shock 70 kDa protein protein, , Chaperone protein dnaK protein, Co chaperone with DnaJ protein, groP protein, HSP70 protein - 分子量
- 17.7 kDa (163 AA)
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