Mast Cell-Degranulating Peptide (MCDP) Peptide
MCDP
适用: Bee
宿主: 合成
BP
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- 抗原
- Mast Cell-Degranulating Peptide (MCDP)
- 蛋白类型
- Synthetic
- 宿主
- Bee
- 资源
- 合成
- 应用范围
- Blocking Peptide (BP)
- 原理
- A Potent and Selective Blocker of KV1.1 and KV1.2 Channels
- 序列
- IKCNCKRHVI KPHICRKICG KN-NH2
- 特异性
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Toxin target: KV1.1, KV1.2 K+ channels,
Disulfide bonds between: Cys3-Cys15, and Cys5-Cys19 Asn22 - C-terminal amidation - 产品特性
- A Potent and Selective Blocker of KV1.1 and KV1.2 Channels
- 纯度
- ≥98 % (HPLC)
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- 应用备注
- Antigen Preadsorption Control: 10 nM - 1 μM
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
- 缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- 抗原
- Mast Cell-Degranulating Peptide (MCDP)
- 背景
- Mast cell degranulating peptide, MCDP, Peptide 401,MCD peptide (Mast Cell Degranulating Peptide) is a 22 amino acid peptidyl toxin isolated from Apis mellifera bee venom1. MCD peptide induces histamine release from mast cells2 and release of glutamate in CA1 region of rat hippocampus in dose-dependent manner3.MCD peptide was shown to block KV1.2 channels in Xenopus oocytes4 and to inhibit KV1.1 (IC50 490 nM) and KV1.2 (IC50 440 nM) expressed in cell lines while having no effect on KV1.3, KV1.5 and KV3.15. In accordance, MCD peptide (6.4 μM) enhances the magnitude of field potentials in CA1 region as recorded in hippocampal slices6 and induces epileptiform seizures and brain damage after intracerebroventricular injection of 200 pM in rats7.
- 分子量
- 2587 Da
- UniProt
- P01499
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