Quick Overview for Hm1a Toxin Peptide (ABIN7582232)
抗原
Hm1a Toxin
宿主
Heteroscodra maculata
资源
合成
应用范围
Blocking Peptide (BP)
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蛋白类型
- Synthetic
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原理
- A Selective and Potent Activator of NaV1.1 Channels and Blocker of KV2 and KV4 Channels
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序列
- ECRYLFGGCS STSDCCKHLS CRSDWKYCAW DGTFS-NH2
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特异性
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Toxin target: NaV1.1, KV2, KV4 channels,
Disulfide bonds between: Cys2-Cys16, Cys9-Cys21 and Cys15-Cys28 Ser35 - C-terminal amidation -
产品特性
- A Selective and Potent Activator of NaV1.1 Channels and Blocker of KV2 and KV4 Channels
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纯度
- ≥98 % (HPLC)
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Hm1a Toxin peptide
适用: Heteroscodra maculata 宿主: 合成 BP, FACS, FM, IF, LCI
Hm1a Toxin peptide适用: Heteroscodra maculata 宿主: 合成 BP
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应用备注
- Antigen Preadsorption Control: 100 nM - 1 μM
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说明
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Electrophysiology
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Hm1a Toxin
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背景
- Delta-theraphotoxin-Hm1a, Delta-TRTX-Hm1a, Heteroscodratoxin-1, HmTx1, Kappa-theraphotoxin-Hm1a, Kappa-TRTX-Hm1a,Hm1a Toxin (δ-TRTX-Hm1a) is a peptide toxin originally isolated from Heteroscodra maculate tarantula venoM The toxin was originally described as a KV2 and KV4 channel blocker1 but has since been found to be a selective and specific activator of NaV1.1 voltage-gated sodium channels2,3. It interacts with extracellular loops connecting transmembrane segments 1-2 and 3-4 in domain IV voltage sensor of the channel to inhibit NaV1.1 fast inactivation2,3. Hm1a inhibits human NaV1.1 channel inactivation expressed in Xenopus oocytes with EC50 value of 38 ± 6 nMNaV1.1 channel is a therapeutic target for brain disorders, such as epilepsy, Alzheimer's disease, and autisM It also contributes to mechanical pain by regulating excitability in a specific subset of sensory neurons within the peripheral nervous systeM
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分子量
- 3996.4 Da
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UniProt
- P60992
抗原
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