ATX-II peptide (Atto 647N)
适用: Anemonia sulcata
宿主: 合成
FACS, FM, IF, LCI
产品编号 ABIN7582138
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for ATX-II peptide (Atto 647N) (ABIN7582138)
抗原
ATX-II
宿主
Anemonia sulcata
资源
合成
应用范围
Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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蛋白类型
- Synthetic
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生物活性
- Active
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标记
- Atto 647N
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原理
- ATX-II-ATTO Fluor-647N
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序列
- GVPCLCDSDG PSVRGNTLSG IIWLAGCPSG WHNCKKHGPT IGWCCKQ-OH
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特异性
- Toxin target: NaV1.1-1.7 voltage-gated sodium channels
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产品特性
- ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is 1-2 Molecules of dye per ATX-II molecule, linked via primary amines.
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Biological Activity Comment
- ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
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ATX-II peptide
适用: Anemonia sulcata 宿主: 合成
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应用备注
- Antigen Preadsorption Control: 0.1 - 1 μM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- ATX-II
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背景
- A fluorescent probe for imaging and sensitive detection of NaV sodium channels
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分子量
- 5561 Da
抗原
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