ATX-II peptide (Atto 647N)
适用: Anemonia sulcata
宿主: 合成
BP, FACS, FM, IF, LCI
Quick Overview for ATX-II peptide (Atto 647N) (ABIN7582138)
抗原
ATX-II
宿主
Anemonia sulcata
资源
合成
应用范围
Blocking Peptide (BP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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蛋白类型
- Synthetic
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标记
- Atto 647N
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原理
- A Fluorescent Conjugate Marker for Sensitive Detection of NaV Channels
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序列
- GVPCLCDSDG PSVRGNTLSG IIWLAGCPSG WHNCKKHGPT IGWCCKQ-OH
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特异性
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Toxin target: Various NaV channels,
Disulfide bonds between: Cys4-Cys44, Cys6-Cys34 and Cys27-Cys45 ATTO Fluor-647N -
产品特性
- A Fluorescent Conjugate Marker for Sensitive Detection of NaV Channels
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ATX-II peptide
适用: Anemonia sulcata 宿主: 合成 BP
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应用备注
- Antigen Preadsorption Control: 0.1 - 1 μM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- -20 °C
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储存方法
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- ATX-II
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背景
- Neurotoxin 2, Toxin ATX-II, As2, Anemonia viridis toxin 2, Av2, Delta-actitoxin-Avd1c 2, Toxin II,ATX-II is a 47 amino acid peptidyl toxin, originally isolated from Anemonia sulcata sea anemone venom. It is a potent neurotoxin, which modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and -insensitive Na+ channels in various excitable tissue and cell types (at concentration range of 10-100 nM)1-9.
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分子量
- 5561 Da
抗原
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