Quick Overview for Conotoxin MVIIC omega Peptide (ABIN7582090)
抗原
Conotoxin MVIIC omega
宿主
Conus magus (Magical cone)
资源
合成
应用范围
Blocking Peptide (BP)
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蛋白类型
- Synthetic
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原理
- A Blocker of Presynaptic CaV (N- and P/Q-Type) Channels
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序列
- CKGKGAPCRK TMYDCCSGSC GRRGKC-NH2
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特异性
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Toxin target: N-type and P/Q-type Ca2+ channels,
Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys26 Cys26 - C-terminal amidation -
产品特性
- A Blocker of Presynaptic CaV (N- and P/Q-Type) Channels
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纯度
- ≥99 % (HPLC)
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Conotoxin MVIIC omega peptide (Biotin)
适用: Conus magus (Magical cone) 宿主: 合成 BP, FACS, FM, IF, LCI
Conotoxin MVIIC omega peptide适用: Conus magus (Magical cone) 宿主: 合成 BP, FACS, FM, IF, LCI
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应用备注
- Antigen Preadsorption Control: 50 nM - 1 μM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Conotoxin MVIIC omega
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背景
- SNX-230,ω-Conotoxin MVIIC blocks CaV2.1 (α1A, P/Q-type) and CaV2.2 (α1B, N-type) channels.1 The toxin binds with high affinity to CaV2.1 and with lower affinity to CaV2.2 in rabbit brain.2 However, the block by ω-Conotoxin-MVIIC of N-type channels in DRG neurons developed much faster than the block of P-type currents in Purkinje cells.1 The effect of the toxin is modulated by voltage (i.e. it is more potent for inactivated channels).3In addition this toxin was reported to block nicotinic receptors (transiently expressed in Xenopus oocytes) with IC50 of 1.3 μM4 It was also shown to inhibit K+-induced 3H-GABA release in hippocampus in vivo.5 This effect was with high affinity (50 % block, 200 nM). The toxin was used to inhibit synaptic transmission in several peripheral preparations.6,7
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分子量
- 2749 Da
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UniProt
- P37300
抗原
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