Methylphosphate Capping Enzyme (MEPCE) Peptide
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北京 101111
Quick Overview for Methylphosphate Capping Enzyme (MEPCE) Peptide (ABIN370222)
抗原
资源
应用范围
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序列
- KQQRKFQYGN YCK
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产品特性
- This peptide is the immunizing peptide for ABIN238571 and may be used for blocking experiments
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纯化方法
- The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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应用备注
- When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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缓冲液
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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储存条件
- -20 °C
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储存方法
- Shipped at ambient temperature, store at -20°C.
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- MEPCE (Methylphosphate Capping Enzyme (MEPCE))
抗原
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