When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
缓冲液
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
储存条件
-20 °C
储存方法
Shipped at ambient temperature, store at -20°C.
抗原
KIF5B
(Kinesin Family Member 5B (KIF5B))
别名
KINH Peptide, KNS Peptide, KNS1 Peptide, UKHC Peptide, Khc Peptide, AL022807 Peptide, Khcs Peptide, Kns1 Peptide, Ukhc Peptide, khc Peptide, KIF5A Peptide, kinesin family member 5B Peptide, kinesin heavy chain Peptide, kinesin-1 Peptide, kinesin-1 heavy chain Peptide, kinesin family member 5B S homeolog Peptide, KIF5B Peptide, LOC411769 Peptide, cgd6_2790 Peptide, kin-1 Peptide, Tb927.1.1350 Peptide, LACBIDRAFT_305999 Peptide, Khc Peptide, LOC100538629 Peptide, Kif5b Peptide, kif5b.S Peptide