Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 °C for overnight or at room temperature for 2 hours.
WIPI49, WD repeat domain phosphoinositide-interacting protein 1, WIPI-1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa