人 CXCL9 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- 抗原 See all CXCL9 products
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
- 抗原表位
- AA 23-125
- 适用
- 人
- 检测方法
- Colorimetric
- 宿主
- 兔
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL9 in Human samples.
- 品牌
- EZ-Set™
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: T23-T125 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 产品特性
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
- 组件
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- 试剂未包括
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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- 说明
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - 限制
- 仅限研究用
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- 缓冲液
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- 有效期
- 12 months
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- 抗原
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
- 别名
- CXCL9 (CXCL9 产品)
- 背景
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Protein Function: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
Background: Chemokine(C-X-C motif) ligand 9(CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon(MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
Synonyms: C-X-C motif chemokine 9,Gamma-interferon-induced monokine,Monokine induced by interferon-gamma,HuMIG,MIG,Small-inducible cytokine B9,CXCL9,CMK, MIG, SCYB9,
Full Gene Name: C-X-C motif chemokine 9
Cellular Localisation: Secreted. - UniProt
- Q07325
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