(4 references)-
- 抗原 See all IL-5 (IL5) products
- IL-5 (IL5) (Interleukin 5 (IL5))
- 适用
- 人
- 应用范围
- ELISpot
- 品牌
- BD™ ELISPOT
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤0.01 ng/μg of protein.
- 组件
- This product contains sufficient reagent for five 96-well plates, including unlabelled capture antibody (no azide/low endotoxin format), biotinylated detection antibody, and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.
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- 应用备注
- The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
- 说明
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BD™ ELISPOT Human IL-5 ELISPOT Pair
- 限制
- 仅限研究用
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- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.
- 储存液
- Without preservative
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).
: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).
: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).
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: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
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- 抗原
- IL-5 (IL5) (Interleukin 5 (IL5))
- 别名
- IL-5 (IL5 产品)
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