Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) 抗体
Quick Overview for Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) 抗体 (ABIN185294)
抗原
See all Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) products适用
宿主
克隆类型
应用范围
质量等级
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抗原表位
- AA 429-442, AA 443-456
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原理
- LXR alpha / LXR beta
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序列
- CRLQDKKLPP LLSEI
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特异性
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
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亚型
- IgG
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应用备注
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Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
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别名
- NR1H3, NR1H2
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背景
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
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基因ID
- 10062, 7376
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NCBI登录号
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
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途径
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
抗原
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