Nitrate Reductase 抗体
The 兔 多克隆 anti-Nitrate Reductase antibody is suitable to detect Nitrate Reductase in samples from Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Gracilaria gracilis (Red alga), Leptodictyum riparium, Mung bean, Panax notoginseng, Phaeodactylum tricornutum, Poplar (Populus), Potato, Thalassiosira pseudonana, Tomato, Trebouxia 和 Vitis vinifera. It has been validated for WB.
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产品编号 ABIN334562
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Nitrate Reductase 抗体 (ABIN334562)
抗原
Nitrate Reductase (NR)
(Nitrate Reductase, Assimilatory (NR))
适用
Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Gracilaria gracilis (Red alga), Leptodictyum riparium, Mung bean, Panax notoginseng, Phaeodactylum tricornutum, Poplar (Populus), Potato, Thalassiosira pseudonana, Tomato, Trebouxia, Vitis vinifera
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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原理
- Anti-NR | Nitrate reductase, assimilatory
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交叉反应 （详细）
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Arabidopsis thaliana, Chlamydomonas reinardtii, red alga Gracilaria gracilis, Hordeum vulgare,Leptodictyum riparium (Hedw.) Warnst (moss), Medicago sativa, Phaeodactylum tricornutum Bohlin accession Pt1 8.6, Panax notoginseng, Populus yunanensis Dode, Solanum lycopersicum, Solanum tuberosum, Thalassiosira sp. (diatom), Trebouxia sp.,Vigna radiata, Vitis vinigera
Not reactive in: Aspergilus niger, Emiliania huxleyi, Tisochrysis lutea -
预测反应
- Arabis alpina,Brachypodium distachyon,Brassica napus,Brassica rapa subsp. pekinensis,Capsella rubella,Citrus clementina,Citrus sinensis,Chlorella vulgaris,Dunaliella salina,marine Diatoms,Coffea canephora,Eucalyptus grandis,Glycine max,Glycine soja,Gossypium arboretum,Helianthus annuus,Lycopersicum esculentum,Morus alba,Nannochloropsis gaditana,Nicotiana tabacum,Nicotiana attenuata,Nicotiana benthamiana,Oryza sativa,Phaseolus vulgaris,Phytophthora infestans,Physcomitrium patens,Prunus persica,Ricinus communis,Sorghum bicolor,Spinacia oleracea,Solanum lycopersicum,Symbiodinium microadriaticum,Theobroma cacao,Zea mays
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832
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应用备注
- 1 : 500 -1 : 1000 (WB)
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说明
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ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.For working with diatom samples ECL Advance (GE Healthcare) or other more sensitive ECL detection reagent is recommended.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
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背景
- Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
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分子量
- 103 kDa | 117 kDa
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基因ID
- 844112
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UniProt
- P11832, P11035
抗原
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