A431+CAL A/OKA ACID (2HR) Lysate
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北京 101111
Quick Overview for A431+CAL A/OKA ACID (2HR) Lysate (ABIN968887)
应用范围
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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资源
- 人细胞
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产品特性
- BD Transduction Laboratories™ A431 + Calyculin A/Okadaic Acid Cell Lysate
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纯化方法
- Purified
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NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
NXNL1 293T Cell Transient Overexpression Lysate(Denatured)NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
LOC124220 293T Cell Transient Overexpression Lysate(Denatured)ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
TMEM42 293T Cell Transient Overexpression Lysate(Denatured)TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
SIRPD 293T Cell Transient Overexpression Lysate(Denatured)SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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实验流程
- Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. A431 cells were grown to 80-90 % confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37 °C.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)
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储存条件
- -20 °C
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储存方法
- Do not dilute or boil. Store undiluted at -20° C.
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