HeLa whole-cell extract (TNF-α stimulated)
-
- 资源
- 人细胞
- 产品特性
-
Origin: Human Cervix Adenocarcinoma
- 组件
- 2 x 125 μg of HeLa whole-cell extract (TNF-α stimulated) at 2.50 μg/μl
- Lysed Cells
- HeLa Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
- Lysate Treatment
- TNF-α stimulated
-
-
- 限制
- 仅限研究用
-
- 浓度
- 2.50 μg/μL
- 缓冲液
- The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
- 注意事项
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- To ensure stability, extracts should be stored at -80°C.
-