DYKDDDDK Positive Control Lysate
PC, WB
Whole Cell Lysate
Overexpression Lysate
E. coli
适用: 标记
Quick Overview for DYKDDDDK Positive Control Lysate (ABIN1607004)
抗原
DYKDDDDK Tag
宿主
标记
应用范围
Positive Control (PC), Western Blotting (WB)
Lysed Cells
E. coli
Lysate Type
Overexpression Lysate
Lysate Fraction
Whole Cell Lysate
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资源
- 大肠杆菌（E. Coli）
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Supplier Product No.
- w00-001-383
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Supplier
- Rockland
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内毒素水平
- Low Endotoxin : No
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应用备注
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
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储存条件
- -80 °C
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储存方法
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
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有效期
- Expiration date is three (3) months from date of opening.
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- DYKDDDDK Tag
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物质类
- Tag
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背景
- This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
抗原
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