Cryptosporidium ELISA 试剂盒
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Quick Overview for Cryptosporidium ELISA 试剂盒 (ABIN997059)
抗原
适用
检测方法
实验类型
应用范围
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原理
- This ELISA is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces.
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Analytical Method
- Qualitative
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特异性
- 100 %
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样本量
- 1 g
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实验时间
- 1 - 2 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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: "Prevalence of Cryptosporidium infection and related risk factors in children in Awo and Iragberi, Nigeria." in: Journal of immunoassay & immunochemistry, (2016) (PubMed).
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- Cryptosporidium
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物质类
- Parasite
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背景
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Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men.
Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. One important alternative has been the development of an antigen capture enzyme linked immunosorbent assay (ELISA) for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.
抗原
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