Schistosoma ELISA 试剂盒

Quality Control:
The use of a positive and negative control allows easy validation of kit stability. For a valid test, the positive control must be over 0.5 OD units and the negative control must be under 0.2 units. Should the values fall outside these ranges, the kit should not be used.
Limitations of procedure: Serological results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.

Preparation Wash Buffer - Remove cap and add contents of bottle to 475 mL DI water. Place diluted wash buffer into a squeeze bottle.
Note:
Washings consist of filling to the top of each well, shaking out the contents and refilling. Avoid generating bubbles in the wells during the washing steps. Test samples: Make a 1:40 dilution of patients' sera using the dilution buffer.

Serological specimens should be collected under aseptic conditions. Coagulated blood and removed serum. Hemolysis is avoided through prompt separation of the serum from the clot. Serum should be stored 2-8 °C if it is to be analyzed within a few days. Serum may be held for 3 to 6 months by storage at -20 °C or lower. Lipemic and strongly hemolytic serum should be avoided. Do not heat inactivate serum. Avoid repeated freezing and thawing of samples. AccuDiag™ Schistosoma IgG ELISA Kit

1. Break off number of wells needed (two for controls plus number of samples) and place in strip holder.
   2. Add 100 μL of negative control to well #1, 100 μL of positive control to well #2, and 100 μL of the diluted (1:40) test samples to the remaining wells. Note: Negative and positive controls are supplied as prediluted. Do not dilute.
   3. Incubate at room temperature (15 °C - 20 °C) for 10 minutes.
   4. Shake out contents and wash 3 times with diluted wash buffer.
   5. Add 100 μL of enzyme conjugate to each well.
   6. Incubate at room temperature for 10 minutes.
   7. Shake out contents and wash 3 times with wash buffer.
   8. Add 100 μL of Chromogen to every well.
   9. Incubate at room temperature for 5 minutes.
   10. Add 100 μL of stop solution.
   11. Zero ELISA reader on air, read wells at 450 nm with a reference filter at 620650 nm or read results visually.
   Washings consist of using the diluted wash buffer to fill to the top of each well, shaking out the contents and refilling the wells for a total of 3 times. Avoid generating bubbles in the wells during the washing steps. Controls must be included each time the kit is run.
   2. Squeeze bottle containing diluted wash buffer may be stored at room temperature.

Spectrophotometer: Zero ELISA reader on air. Read all wells using a bichromatic reading with filters at 450 nm and 620-650 nm. Positive - Absorbance reading greater or equal to 0.2 OD units. Negative - Absorbance reading less than 0.2 OD units.