Mumps IgM ELISA 试剂盒
适用: Mumps Virus
Colorimetric
Competition ELISA
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- 抗原
- Mumps IgM
- 适用
- Mumps Virus
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 原理
- Mumps IgM Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the qualitative detection of IgM antibody in human serum to Mumps for the determination of immunological experience.
- Analytical Method
- Qualitative
- 特异性
- 96.60%
- 灵敏度
- 99.30%
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- 样本量
- 10 μL
- 实验时间
- 1 - 2 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12-14 months
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- 抗原
- Mumps IgM
- 物质类
- Antibody
- 背景
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The mumps virus is a member of the paramyxovirus group and the etiological agent of mumps in man. Mumps is a generalized illness usually accompanied by parotid (salivary gland) swelling and mild symptoms. It is also one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries.
Parotitis as a presenting symptom in mumps infections is usually sufficiently diagnostic to preclude serological confirmation. However, a third of mumps infections are subclinical or unrecognized and may require viral isolation and/or some other serological procedure to confirm or rule out mumps infection. An example of this is presenting orchitis or meningoencephalitis, the two most common complications of mumps infection, without salivary gland involvement.Virus isolation is time consuming and cumbersome and is usually an impractical procedure for the typical clinical laboratory. Current methods for serodiagnosis of mumps infections are in-vitro serum neutralization, hemagglutination-inhibition (HAI), indirect immunofluorescence, and complement fixation (CF) tests. Of these methods, neutralization is reportedly the most specific. However, the neutralization test requires 4-5 days to complete the test. HAI and CF are reportedly less sensitive than the neutralization test. These methods lack specificity, which limits their usefulness in determining immune status. The HAI test also requires pretreatment of test sera to remove nonspecific hemagglutination inhibitors from some sera.
Infection with mumps virus, whether symptomatic or subclinical, is generally thought to offer lifelong immunity. As first described by Engvall and Perlman and Van Weeman, Enzyme Immunoassays can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of enzyme-linked immunoassays can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization.
ELISA is as sensitive as the neutralization test and more sensitive than CF and HAI which makes it a reliable test for determination of immune status.
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