Testosterone ELISA 试剂盒
Quick Overview for Testosterone ELISA 试剂盒 (ABIN996933)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0 ng/mL
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原理
- Testosterone Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the quantitative determination of testosterone in human serum.
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Analytical Method
- Quantitative
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特异性
- 100%
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灵敏度
- 0.05 ng/mL
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样本量
- 10 μL
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实验时间
- 1 - 2 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12-14 months
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- Testosterone
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物质类
- Hormone
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背景
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Testosterone (17 beta -hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the beta position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes, in females ca. 50 % of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25 % from the ovary and ca. 25 % from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer.
Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter?s syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases.The Testosterone EIA kits are designed for the measurement of total Testosterone in human serum.
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