ENO2/NSE ELISA 试剂盒
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Quick Overview for ENO2/NSE ELISA 试剂盒 (ABIN996903)
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See all ENO2/NSE (ENO2) ELISA试剂盒检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0 ng/mL
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原理
- The Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.
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Analytical Method
- Quantitative
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特异性
- 98.7 %
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灵敏度
- 15 ng/mL
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Enolase 2 (Gamma, Neuronal) (ENO2) ELISA Kit
ENO2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 人 Colorimetric Sandwich ELISA 1.2-178 ng/mL Serum
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 兔 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 绵羊 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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样本量
- 15 μL
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实验时间
- 1 - 2 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12-14 months
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE
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背景
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The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes ( alphaalpha , alphabeta alphagamma and gammagamma ) composed of three distinct subunits, alpha , beta , and gamma . Three isoenzymes are found in human brain: alphaalpha , alphagamma and gammagamma . The alphagamma and gammagamma -enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population.
Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of alphagamma and gammagamma isoenzyme. The determination of NSE should detect and gamma isoforms with the same sensitivity. The antibodies for this particular assay are specific for the gamma -subunit without cross reactivity with alpha or beta subunits. NSE Kits are reported to be useful diagnostic marker for lung cancer, neuroblastoma, and melanoma, seminoma (S) and in injury of central nervous system. In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.
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