dsDNA Ab ELISA 试剂盒
This 人 dsDNA Ab ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 dsDNA Ab. This ELISA Kit has been cited in1 publication.
(1 reference)Quick Overview for dsDNA Ab ELISA 试剂盒 (ABIN996874)
抗原
See all dsDNA Ab products
dsDNA Ab
(Anti-Double Strand DNA Antibody (dsDNA Ab))
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
样品类型
Serum
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原理
- dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).
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Analytical Method
- Semi-Quantitative
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特异性
- 97.9 %
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灵敏度
- 100%
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组件
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1. Microplate: wells coated with Purified dsDNA antigen
2. Serum Diluent Type II
3. Positive Control: Human serum or defibrinated plasma
4. Calibrator: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Goat anti-human IgG and IgM
7. Wash Buffer Type I 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: 1N H2SO4 solution -
试剂未包括
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1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)
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应用备注
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Notable features of Autoimmune Disease ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results -
样本量
- 10 μL
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实验时间
- 1.5 h
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板类型
- Pre-coated
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实验流程
- dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes.
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12-14 months
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: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).
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: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).
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- dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))
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别名
- DsDNA Antibody
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物质类
- Antibody, Antibody
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背景
- Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome.
抗原 See all dsDNA Ab products
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