Urinary Diacetylspermine ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of Rodents Urinary Diacetylspermine.
产品编号 ABIN956345
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for Urinary Diacetylspermine ELISA 试剂盒 (ABIN956345)
抗原
Urinary Diacetylspermine
适用
Rodents
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
样品类型
Urine
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原理
- The Urinary Diacetylspermine EIA is for the quantitative determination of urinary diacetylspermine in rodents.
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Analytical Method
- Quantitative
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产品特性
- Our kit is convenient for use quantifying the amount of urinary diacetylspermine by using the ELSA method. This kit is for research use only. Highly sensitive and specific Strip type well, antigen pre-coated microplate. Assay range: 6.25-200 nM
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组件
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Wash Solution: 30 mL 20x concentrate
Antibody Diluent: 20 mL Antibody
Plate: 96 wells
Calibrator: 2 x 250 µL Anti-Diacetylspermine
Antibody: 60 µL 100x concentrate
HRP-Anti Rabbit IgG Antibody: 80 µL 80x concentrate
OPD (o-phenylendiamine) tablets x2
Substrate Solution: 30 mL
Stop Solution: 15 mL
Dilution Plate: 1 plate. -
试剂未包括
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Micropipette and tip
Plate washer
Plate reader
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板类型
- Pre-coated
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样品制备
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- Collect urine in sampling tube. Add 0.1% Na2N3 at final concentration.
2. After centrifugation at 1500 rpm for 5 minutes, dilute the resulting supernatant over 4 times with distilled water.
3. Measure the amount of creatinine in the remaining diluted supernatant for compensation.
4. Prepared urine should be kept below -30°C if necessary.
Procedure: Pre-reaction: Prepare the calibrator control wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of the 6 calibrators (200, 100, 50, 25, 12.5, 6.25 nM) in the dilution plate. Likewise prepare the experimental wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of prepared urinary sample in the same plate. After settlement, incubate at room temperature for 1 hour.
- Collect urine in sampling tube. Add 0.1% Na2N3 at final concentration.
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结果分析
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In general, the concentration of urine changes easily by the amount of water taken in or by the environment. The amount of urinary creatinine depends on the amount of muscle and their measurements correlate positively.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- All reagents should be stored at -30°C.
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- Urinary Diacetylspermine
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背景
- Polyamines are generally believed to function both in protein synthesis and DNA synthesis leading to control cell proliferation. It is reported that the total amount of urinary polyamines are elevated in cancer patients. Assays have already been developed to measure the total amount of these urinary polyamines. Recently two diacetyl-derivatives, N1, N12-diacetylspermine and N1, N8-diacetylspermine, were found to correlate to the status of disease more closely than the total amount of polyamines.
抗原
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