Quick Overview for Total Antioxidant Capacity ELISA 试剂盒 (ABIN956217)
抗原
Total Antioxidant Capacity
适用
化学剂
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
21.9-4378 μmol/L
应用范围
ELISA
最低检测浓度
21.9 μmol/L
Analytical Method
Quantitative
灵敏度
21.9 μmol/L
产品特性
Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.
组件
1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water. 2. Sample diluent: 60 mL x 1 bottle, Ready to use. 3. Cu++ solution: 5 mL x 1 bottle, Ready to use. 4. Stop solution: 5 mL x 1 bottle, Ready to use. 5. Microtiter plate: 1 plate
试剂未包括
A. A microplate reader (measuring wavelength 490 nm) B. Pipettes and pipette chips C. Plastic test tubes D. Distilled water E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).