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CRP ELISA 试剂盒

Name: CRP ELISA 试剂盒
SKU: ABIN956163
Availability: OutOfStock

This 马 CRP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 马 CRP.

产品编号 ABIN956163

发货至: 中国

Quick Overview for CRP ELISA 试剂盒 (ABIN956163)

抗原

See all CRP ELISA试剂盒

CRP (C-Reactive Protein (CRP))

适用

马

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

Discover our top product CRP ELISA Kit

原理

The Horse CRP ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative determination of CRP in horse biological samples.

Analytical Method

Quantitative

产品特性

In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.

组件

1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-horse CRP antibody conjugated with HRP in stabilizing buffer.
4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-horse CRP.
7. Horse CRP Calibrator: One vial containing a Horse CRP Calibrator.

试剂未包括

Precision pipettes (2 µL to 200 µL) for making and dispensing dilutions
Test tubes
Microplate washer/aspirator
Distilled or de-ionized H2O Microplate reader
Assorted glassware for the preparation of reagents and buffer solutions
Timer
Vortex mixer
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板类型

Pre-coated

试剂准备
1. Diluent Concentrate: The Diluent solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
  2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30-35°C before dilution can dissolve crystals.
  3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
  4. TMB Substrate Solution: Ready to use as supplied.
  5. Stop Solution: Ready to use as supplied.
  6. Microtiter Plate: Ready to use as supplied. Unseal microtiter pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal.
  7. Horse CRP Calibrator: The Horse CRP Calibrator should be aliquoted out and stored frozen. The calibrator is now at a concentration of 4.837 µg/mL and needs to be diluted in 1X diluent according to the chart below for each run. Horse CRP Calibrators need to be prepared immediately prior to use. Mix well between each step. Avoid foaming.
结果分析
1. Subtract the average background value from the test values for each sample.
  2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve, although a second order polynomial (quadratic) or other curve fits may also be used.
  3. Interpolate test sample values from calibration curve. Correct for sample dilution factor to arrive at CRP concentration in original sample.
  
  1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
  2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water, and accuracy of reagent and sample pipetting, washing technique, incubation time or temperature.
限制

仅限研究用
储存条件

4 °C

储存方法

1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long term storage recommendations below for the Horse CRP Calibrator.
2. Diluent: The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
3. Wash Solution: The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16-25°C) or at 4°C.
4. Enzyme-Antibody Conjugate: Undiluted anti-CRP-HRP conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
7. Microtiter Plate: Anti-horse CRP coated wells are stable until the expiration date, and should be stored at 4°C in the sealed foil pouch with desiccant pack
8. Horse CRP Calibrator: Long term storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until the expiration date. Short term storage: The calibrator is stable for up to 14 days at 4°C. The working calibrator solutions should be prepared immediately prior to use and are stable for up to 8 hours.

有效期

The expiry date is stated on the label.
抗原 See all CRP ELISA试剂盒

CRP (C-Reactive Protein (CRP))

别名

CRP

背景

Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 1930. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in humans, is normally present as a trace constituent of serum at levels less than 0.3 mg/dL. The levels in serum rise quickly following acute tissue damage and also fall very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well.

途径

Carbohydrate Homeostasis