FABP1 ELISA 试剂盒
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北京 101111
Quick Overview for FABP1 ELISA 试剂盒 (ABIN956112)
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See all FABP1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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特异性
- Cross-reactivity with intestinal FABP has not been investigated.
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产品特性
- The Rat L-FABP ELISA is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses affinity purified anti-rat L-FABP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-rat L-FABP antibodies for detection. The test sample is diluted and incubated with conjugate in the microtiter wells for 60 minutes. This results in L-FABP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of L-FABP is proportional to the optical density of the test sample and the actual value is derived by reference to a calibration curve.
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组件
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Anti-rat L-FABP antibody coated microtiter plate with 96 wells (provided as 12 detachable strips of 8)
Enzyme Conjugate Reagent, 11 mL
Rat L-FABP Calibrator (lyophilized), containing 2 µg/mL rat L-FABP when reconstituted as detailed on the vial label
Diluent, 25 mL
Wash Solution (20X), 50 mL
TMB Reagent (One-Step), 11 mL
Stop Solution (1N HCl), 11 mL. -
试剂未包括
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Precision pipettes and tips
Distilled or de-ionized water
Polypropylene or glass tubes
Vortex mixer
Absorbent paper or paper towels
Micro-Plate incubator/shaker with an approximate mixing speed of 150 rpm
A microtiter plate reader at 450 nm wavelength, with a bandwidth of 10 nm or less and an OD range of 0-4 OD
Graph paper (PC graphing software is optional)
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Fatty Acid Binding Protein 1, Liver (FABP1) ELISA Kit
FABP1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitHigh Sensitivity FABP1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: 小鸡 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitWide Range FABP1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: Cow Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 1, Liver (FABP1) ELISA KitFABP1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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样品制备
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Serum samples may be tested undiluted or after dilution with diluent. The optimal dilution factor should be determined empirically.
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实验流程
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- Secure the desired number of coated wells in the holder.
2. Dispense 100 µL of calibrators and diluted samples into the wells (we recommend that samples be tested in duplicate).
3. Add 100 µL of enzyme conjugate reagent into each well.
4. Incubate on an orbital micro-plate shaker at 100-150 rpm at room temperature (18-25°C) for 60 minutes.
5. Wash and empty the microtiter wells 5 times with 1X wash solution. Optimal results are obtained if a plate washer (400 µL/well) is used. However, if a plate washer is not available a squirt bottle may be used to manually wash the wells. The entire wash procedure should be performed as quickly as possible
6. Strike the wells sharply onto adsorbent paper or paper towels to remove all residual droplets.
7. Dispense 100 µL of TMB reagent into each well.
8. Gently mix on an orbital micro-plate shaker at 100-150 rpm at room temperature (18-25°C) for 20 minutes.
9. Stop the reaction by adding 100 µL of Stop Solution to each well.
10. Gently mix. It is important to make sure that all the blue color changes to yellow.
11. Read the optical density at 450 nm with a microtiter plate reader within 5 minutes.
- Secure the desired number of coated wells in the holder.
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结果分析
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- Calculate the average absorbance values (A450) for each set of reference calibrators, and samples.
2. Construct a calibration curve by plotting the mean absorbance obtained from each reference calibrator against its concentration in ng/mL on linear graph paper, with absorbance values on the vertical or Y-axis and concentration on the horizontal or X-axis.
3. Using the mean absorbance value for each sample, determine the corresponding concentration of L-FABP in ng/mL from the calibration curve.
4. Multiply the derived concentration by the dilution factor to determine the actual concentration of L-FABP in the serum/plasma sample.
5. If available, PC graphing software may be used for the above steps.
6. If the OD450 values of samples fall outside of the calibration curve samples should be diluted appropriately and re-tested.
- Calculate the average absorbance values (A450) for each set of reference calibrators, and samples.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- The unused kit should be stored at 4°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable until the expiration date provided that the components are stored as described above.
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有效期
- The expiry date is stated on the label.
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- FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))
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别名
- L-FABP
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背景
- Liver Fatty Acid Binding Protein (L-FABP) has a molecular weight of ~ 14 kDa and constitutes 2-5% of liver cytosolic protein. Immunologically distinct intestinal and cardiac FABP isoforms also exist. All isoforms serve a role in fatty acid transport and metabolism. L-FABP is expressed primarily in the liver but relatively high levels are found in intestinal tissue and it is expressed in other tissues also. L-FABP has recently been identified as a possible biomarker for lung, kidney and liver disease. The L-FABP kit manufactured by does not recognize rat cardiac FABP.
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途径
- Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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