Haptoglobin ELISA 试剂盒
Quick Overview for Haptoglobin ELISA 试剂盒 (ABIN956101)
抗原
See all Haptoglobin (HP) ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
- The Cat Haptoglobin ELISA is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses affinity purified anti-cat haptoglobin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-cat haptoglobin antibodies for detection. The test sample is diluted and incubated in the microtiter wells for 45 minutes. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. This results in haptoglobin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of haptoglobin is proportional to the optical density of the test sample.
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组件
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Anti-cat haptoglobin antibody coated microtiter plate with 96 wells (provided as 12 detachable strips of 8)
Enzyme Conjugate Reagent, 11 mL
Cat Haptoglobin Calibrator (lyophilized)
Wash Buffer (20X), 50 mL
Diluent (10X), 25 mL
TMB Reagent (One-Step), 11 mL
Stop Solution (1N HCl), 11 mL. -
试剂未包括
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Precision pipettes and tips
Distilled or de-ionized water
Polypropylene or glass tubes
Vortex mixer
Absorbent paper or paper towels
Micro-Plate incubator/shaker mixing speed of ~150 rpm
Plate reader with an optical density range of 0-4 at 450 nm
Graph paper (PC graphing software is optional)
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Haptoglobin (HP) ELISA Kit
HP 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: Cow Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHigh Sensitivity HP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: Cow Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Haptoglobin (HP) ELISA KitHP 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Haptoglobin (HP) ELISA KitHP 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Urine
Haptoglobin (HP) ELISA KitHP 适用: 大鼠 Colorimetric Sandwich ELISA 1.95 ng/mL - 125 ng/mL Plasma, Serum, Urine
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- The kit should be stored at 4°C and the microtiter strips should be kept in a sealed bag with desiccant to minimize exposure to damp air. The kit will remain stable until the expiration date provided that the components are stored as described above.
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有效期
- The expiry date is stated on the label.
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- Haptoglobin (HP)
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别名
- Haptoglobin
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背景
- Haptoglobin is an acute phase protein, the concentrations of which can increase two to ten fold in cat serum as a result of disease. Measurement of haptoglobin provides a convenient marker of inflammation and disease in cats.
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途径
- Transition Metal Ion Homeostasis
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