电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Hemopexin ELISA 试剂盒

HPX 适用: 小鼠 Colorimetric Sandwich ELISA
产品编号 ABIN956032
发货至: 中国
  • 抗原 See all Hemopexin (HPX) ELISA试剂盒
    Hemopexin (HPX)
    适用
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    The Mouse Hemopexin ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative determination of hemopexin in mouse serum or plasma.
    Analytical Method
    Quantitative
    产品特性
    In this assay the hemopexin present in the sample reacts with the anti-HX antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound sample proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. This HRP-conjugated antibody forms a complex with the previously bound hemopexin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme is proportional to the concentration of hemopexin in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of hemopexin in the test sample. The quantity of hemopexin in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.
    组件
    1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
    2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
    3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-Mouse hemopexin antibody conjugated with HRP in a stabilizing buffer.
    4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
    5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
    6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-Mouse Hemopexin.
    7. Mouse Hemopexin Calibrator: One vial containing a lyophilized Mouse Hemopexin Calibrator.
    8. Positive Control: One vial containing 50 µL of serum with 0.1% sodium azide. See the Control Certificate for the concentration.
    试剂未包括
    Test tubes
    Precision pipette (2 µL to 200 µL) for making and dispensing dilutions
    Microplate washer/aspirator
    Distilled or de-ionized H2O Microplate reader
    Assorted glassware for the preparation of reagents and buffer solutions
    Timer
    Featured
    Discover our best selling HPX ELISA Kit
    Top Product
    Discover our top product HPX ELISA Kit
  • 板类型
    Pre-coated
    试剂准备
    1. Diluent Concentrate: The Diluent Solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
      2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30-35°C before dilution can dissolve crystals.
      3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
      4. TMB Substrate Solution: Ready to use as supplied.
      5. Stop Solution: Ready to use as supplied.
      6. Microtiter Plate: Ready to use as supplied. Unseal Microtiter Pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal along with desiccant.
      7. Mouse Hemopexin Calibrator: Add 1.0 mL of distilled or de-ionized water to the lyophilized Mouse Hemopexin Calibrator and mix gently until dissolved. The calibrator is now at a concentration of 4.8 µg/mL (the reconstituted calibrator should be aliquoted and frozen if future use is intended). Prepare the Mouse Hemopexin Calibrators immediately prior to use according to the table below. Mix well between each step. Avoid foaming.
      8. Positive Control: The concentration and recommended dilution are provided on the Control Certificate. Before use, briefly centrifuge the Positive Control to allow all of the liquid to collect in the bottom of the vial.
    结果分析
    1. Subtract the average background value from the test values for each sample.
      2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve. A second order polynomial (quadratic) or other curve fits may also be used.
      3. Interpolate test sample values from the calibration curve. Correct for sample dilution factor to arrive at hemopexin concentration in original sample.

      1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
      2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water and accuracy of reagent and sample pipetting, washing technique, incubation time or temperature.
    限制
    仅限研究用
  • 储存条件
    4 °C
    储存方法
    1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long-term storage recommendations below for the Mouse Hemopexin Calibrator and Positive Control.
    2. Diluent: Concentrate The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
    3. Wash Solution: Concentrate The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16-25°C) or at 4°C.
    4. Enzyme-Antibody Conjugate: Undiluted anti-HX-HRP conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
    5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
    6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
    7. Microtiter Plate: Anti-Mouse Hemopexin coated wells are stable until the expiration date and should be stored at 4°C in the sealed foil pouch with a desiccant pack.
    8. Mouse Hemopexin Calibrator: The lyophilized Mouse Hemopexin Calibrator should be stored at 4°C or frozen until reconstituted. The reconstituted calibrator should be aliquoted and stored frozen. Avoid multiple freeze/thaw cycles. The working calibrator solutions should be prepared immediately prior to use and are stable for up to eight hours.
    9. Positive Control: For storage longer than 7 days keep frozen until the expiration date. Storage less than 7 days can be at 4°C. Avoid multiple freeze/thaw cycles.
    有效期
    The expiry date is stated on the label.
  • 抗原 See all Hemopexin (HPX) ELISA试剂盒
    Hemopexin (HPX)
    别名
    Hemopexin (HPX 产品)
    别名
    HPX ELISA Kit, HX ELISA Kit, Hpxn ELISA Kit, hx ELISA Kit, wu:fi17f11 ELISA Kit, wu:fi19a10 ELISA Kit, hemopexin ELISA Kit, HPX ELISA Kit, Mmar10_1124 ELISA Kit, Hpx ELISA Kit, hpx ELISA Kit
    背景
    Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure hemopexin in serum, tissue extracts and other biological fluids.
    途径
    Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
You are here:
客服