MMP7 ELISA 试剂盒
-
- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
-
适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Rat MMP7 in serum, plasma and other biological fluids.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Rat MMP7. No significant cross- reactivity or interference between Rat MMP7 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between Rat MMP7 and all the other analogues, therefore, cross reaction may still exist.
- 灵敏度
-
The minimum detectable dose of Rat MMP7 is typically less than 0.046 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP7. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of MMP7 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
-
Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
-
1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling MMP7 ELISA Kit
- Top Product
- Discover our top product MMP7 ELISA Kit
-
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
MMP7 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 马 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 山羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 AA 18-267 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 total Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
SensoLyte® 520 MMP-7 Assay KitFRET Fluorometric
-
- 说明
-
The calibration curve concentrations used for the ELISA's were 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156 ng/mL.
- 板类型
- Pre-coated
- 结果分析
-
Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create the calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the MMP7 concentrations versus the log of the OD and the best fit line can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
-
- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
- 别名
- MMP7 (MMP7 产品)
- 别名
- MAT ELISA Kit, MPMM ELISA Kit, MMP-7 ELISA Kit, MPSL1 ELISA Kit, PUMP-1 ELISA Kit, matrilysin ELISA Kit, LOC727698 ELISA Kit, MMP7 ELISA Kit, Matrilysin ELISA Kit, matrix metallopeptidase 7 ELISA Kit, MMP7 ELISA Kit, Mmp7 ELISA Kit
- 途径
- Production of Molecular Mediator of Immune Response
-
