LDL ELISA 试剂盒
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北京 101111
Quick Overview for LDL ELISA 试剂盒 (ABIN955928)
抗原
See all LDL ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 123.5 ng/mL
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原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat LDL in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of rat LDL. No significant cross- reactivity or interference between rat LDL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between rat LDL and all the analogues, therefore, cross reaction may still exist.
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灵敏度
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The minimum detectable dose of rat LDL is typically less than 47.2 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. -
产品特性
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for rat LDL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled rat LDL and unlabeled rat LDL (Calibrators or samples) with the pre-coated antibody specific for rat LDL. After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of LDL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LDL in the sample.
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组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A 1 x 120 µL
Assay Diluent A (2X concentrate) 1 x 6 mL
TMB Substrate 1 x 9 mL
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). -
试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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Low Density Lipoprotein (LDL) ELISA Kit
LDL 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 小鼠 Colorimetric Competition ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitHigh Sensitivity LDL 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 小鼠 Colorimetric Sandwich ELISA 7.81 ng/mL - 500 ng/mL Cell Culture Supernatant, Plasma, Serum
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 大鼠 Colorimetric Competition ELISA 0.312 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 人 Colorimetric Sandwich ELISA 6.25 nmol/mL - 400 nmol/mL Plasma, Serum, Tissue Homogenate
Low Density Lipoprotein (LDL) ELISA KitLDL 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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The calibration curve concentrations used for the ELISA's were 10, 000 ng/mL, 3, 333.3 ng/mL, 1111.1 ng/mL, 370.4 ng/mL, 123.5 ng/mL.
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板类型
- Pre-coated
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结果分析
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This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between LDL concentration in the sample and the assay signal intensity. Low levels of LDL result in a high OD value, while high concentration of LDL results in a low signal. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log graph paper, with LDL concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
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有效期
- The expiry date is stated on the label.
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- LDL (Low Density Lipoprotein (LDL))
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别名
- LDL
抗原 See all LDL ELISA试剂盒
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