Ki-67 ELISA 试剂盒
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北京 101111
Quick Overview for Ki-67 ELISA 试剂盒 (ABIN955919)
抗原
See all Ki-67 (MKI67) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.312 ng/mL
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原理
- This ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human Ki-67P in tissue homogenates, cell lysates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of human Ki-67P. No significant cross-reactivity or interference was observed.
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灵敏度
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The minimum detectable dose of human Ki-67 P is typically less than 0.079 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by the mean O.D. value of 20 replicates of the zero calibrator plus three standard deviations. -
产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67P. Calibrators and samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ki-67P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the TMB substrate solution is added to each well. Only those wells that contain Ki-67P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Ki-67P in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
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组件
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Pre-coated, ready to use 96-well plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). -
试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution
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Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA Kit
MKI67 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitWide Range MKI67 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) ELISA KitMKI67 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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The calibration curve concentrations used for the ELISA's were 20, 10, 5, 2.5, 1.25, 0.625, 0.312 pg/mL.
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板类型
- Pre-coated
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结果分析
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Average the duplicate readings for each calibrator, control, and sample and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Ki-67P concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. It is recommended to use some related software to do this calculation. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- All reagents should be stored according to their label. The Calibrators, Detection Reagent A, Detection Reagent B and the 96-well plate should be stored at -20° C upon receipt. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date, provided they are stored as above.
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有效期
- The expiry date is stated on the label.
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- Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
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别名
- Ki67P
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途径
- Glycosaminoglycan Metabolic Process
抗原 See all Ki-67 (MKI67) ELISA试剂盒
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