IGF2 ELISA 试剂盒
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- 抗原 See all IGF2 ELISA试剂盒
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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适用
- Cow
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.12-200 ng/mL
- 最低检测浓度
- 3.12 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of bovine IGF2 in serum, plasma and other biological fluids.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of bovine IGF2. No significant cross- reactivity or interference between bovine IGF2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between bovine IGF2 and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
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The minimum detectable dose of bovine IGF2 is typically less than 1.24 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF2. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of IGF2 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 说明
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The calibration curve concentrations used for the ELISA's were 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL.
- 板类型
- Pre-coated
- 结果分析
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Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with IGF2 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
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- 抗原 See all IGF2 ELISA试剂盒
- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
- 别名
- IGF2 (IGF2 产品)
- 别名
- C11orf43 ELISA Kit, IGF-II ELISA Kit, PP9974 ELISA Kit, LOC100136550 ELISA Kit, igf2 ELISA Kit, igf2b ELISA Kit, wu:fc26e03 ELISA Kit, zgc:91781 ELISA Kit, igf-2 ELISA Kit, insigf ELISA Kit, pp9974 ELISA Kit, AL033362 ELISA Kit, Igf-2 ELISA Kit, Igf-II ELISA Kit, M6pr ELISA Kit, Mpr ELISA Kit, Peg2 ELISA Kit, IGFII ELISA Kit, RNIGF2 ELISA Kit, IGF-II-B ELISA Kit, igf2-a ELISA Kit, igf2-b ELISA Kit, insulin like growth factor 2 ELISA Kit, insulin-like growth factor II ELISA Kit, insulin-like growth factor 2a ELISA Kit, insulin like growth factor 2 S homeolog ELISA Kit, insulin-like growth factor 2 ELISA Kit, insulin-like growth factor 2 (somatomedin A) ELISA Kit, insulin like growth factor 2 L homeolog ELISA Kit, IGF2 ELISA Kit, LOC100136550 ELISA Kit, igf2a ELISA Kit, igf2.S ELISA Kit, Igf2 ELISA Kit, igf2.L ELISA Kit
- 途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
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