DNAJB1 ELISA 试剂盒
-
- 抗原 See all DNAJB1 ELISA试剂盒
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human HSP40 in tissue homogenates and other biological fluids.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of human HSP40. No significant cross-reactivity or interference between human HSP40 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human HSP40 and all the other analogues, therefore, cross reaction may still exist.
- 灵敏度
-
The minimum detectable dose of human HSP40 is typically less than 0.043 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP40. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of HSP40 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
-
Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
-
1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling DNAJB1 ELISA Kit
- Top Product
- Discover our top product DNAJB1 ELISA Kit
-
DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) ELISA Kit
DNAJB1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) ELISA KitDNAJB1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) ELISA KitDNAJB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 说明
-
The calibration curve concentrations used for the ELISA's were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL.approximate value:
- 板类型
- Pre-coated
- 结果分析
-
Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with HSP40 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
-
- 抗原 See all DNAJB1 ELISA试剂盒
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
- 别名
- Heat Shock Protein 40 (HSP40) (DNAJB1 产品)
- 别名
- HSPF1 ELISA Kit, Hdj1 ELISA Kit, Hsp40 ELISA Kit, RSPH16B ELISA Kit, Sis1 ELISA Kit, CG10578 ELISA Kit, DNAJ-1 ELISA Kit, DNAJ1 ELISA Kit, DROJ1 ELISA Kit, Dmel\\CG10578 ELISA Kit, DnaJ1 64EF ELISA Kit, DroJ1 ELISA Kit, EU3500 ELISA Kit, HSP40 ELISA Kit, HSP40/HDJ1 ELISA Kit, Hsp-40 ELISA Kit, anon-WO0140519.166 ELISA Kit, anon-WO0172774.135 ELISA Kit, dHDJ1 ELISA Kit, dHDJ1/HSP40 ELISA Kit, dHdj1 ELISA Kit, dhdJ1 ELISA Kit, dhdj-1 ELISA Kit, dhdj1 ELISA Kit, dnaJ-1 ELISA Kit, droj1 ELISA Kit, hsp40 ELISA Kit, dnajb4 ELISA Kit, hdj1 ELISA Kit, hspf1 ELISA Kit, sis1 ELISA Kit, DnaJ-5 ELISA Kit, MAS5 ELISA Kit, dnaJ ELISA Kit, 0610007I11Rik ELISA Kit, dnajb1 ELISA Kit, zf-Hsp40 ELISA Kit, zgc:101068 ELISA Kit, DnaJ heat shock protein family (Hsp40) member B1 ELISA Kit, DnaJ-like-1 ELISA Kit, DnaJ (Hsp40) homolog 5 ELISA Kit, type I HSP40 co-chaperone YDJ1 ELISA Kit, DnaJ protein ELISA Kit, molecular chaperone DnaJ ELISA Kit, DnaJ (Hsp40) homolog, subfamily B, member 1a ELISA Kit, DNAJB1 ELISA Kit, Dnajb1 ELISA Kit, DnaJ-1 ELISA Kit, dnajb1 ELISA Kit, DnaJ-5 ELISA Kit, YDJ1 ELISA Kit, dnaJ ELISA Kit, dnajb1a ELISA Kit
-
