Ghrelin ELISA 试剂盒
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Quick Overview for Ghrelin ELISA 试剂盒 (ABIN955873)
抗原
See all Ghrelin (GHRL) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 123.5 pg/mL
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原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human GHRL in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of human GHRL. No significant cross-reactivity or interference between human GHRL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human GHRL and all the analogues, therefore, cross reaction may still exist.
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灵敏度
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123.5 - 10000 pg/mL
The minimum detectable dose of human GHRL is typically less than 45.3 pg/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. -
产品特性
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for human GHRL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled human GHRL and unlabeled human GHRL (Calibrators or samples) with the pre-coated antibody specific for human GHRL. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GHRL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GHRL in the sample.
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组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). -
试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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Ghrelin (GHRL) ELISA Kit
GHRL 适用: 人 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: Pig Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Ghrelin (GHRL) ELISA KitGHRL 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 绵羊 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 兔 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ghrelin (GHRL) ELISA KitGHRL 适用: 小鸡 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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说明
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The calibration curve concentrations used for the ELISA's were 10, 000 pg/mL, 3, 333.3 pg/mL, 1111.1 pg/mL, 370.4 pg/mL, 123.5 pg/mL.
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板类型
- Pre-coated
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结果分析
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This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between GHRL concentration in the sample and the assay signal intensity. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log or semi-log graph paper, with GHRL concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
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有效期
- The expiry date is stated on the label.
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