CRP ELISA 试剂盒
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北京 101111
Quick Overview for CRP ELISA 试剂盒 (ABIN955836)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 46.88 pg/mL
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原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of bovine CRP in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of bovine CRP. No significant cross- reactivity or interference between bovine CRP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between bovine CRP and all the analogues, therefore, cross reaction may still exist.
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灵敏度
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The minimum detectable dose of bovine CRP is typically less than 22.15 pg/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. -
组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). -
试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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C-Reactive Protein (CRP) ELISA Kit
CRP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitHigh Sensitivity CRP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitCRP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
C-Reactive Protein (CRP) ELISA KitHigh Sensitivity CRP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 豚鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: Cow Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
C-Reactive Protein (CRP) ELISA KitCRP 适用: Pig Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
C-Reactive Protein (CRP) ELISA KitCRP 适用: 小鸡 Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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The calibration curve concentrations used for the ELISA's were 3, 000 pg/mL, 1500 pg/mL, 750 pg/mL, 375 pg/mL, 187.5 pg/mL, 93.75 pg/mL, 46.88 pg/mL.
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板类型
- Pre-coated
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结果分析
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Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with CRP concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
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有效期
- The expiry date is stated on the label.
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- CRP (C-Reactive Protein (CRP))
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背景
- The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to CRP. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
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途径
- Carbohydrate Homeostasis
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