Nucleosomes ELISA 试剂盒

AntiNucleosome is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against Nucleosome. The assay is based on microplates coated with human Nucleosomes. The microplate can be divided into 12 modules of 8 wells each or can be used complete for 96 determinations. During this procedure the binding of present autoantibodies, as well as the formation of thesandwich complexes and enzymatic color reaction take place during three different reaction phases. The amount of colour is directly proportional to the concentration of IgG present in the original sample. The optical density of the controls serve as reference for the determination of the cutoff OD. Patent results may be classified in terms of negative, positive or strong positive. Alternatively the concentrations of the controls may be used to calculate a calibration curve for semiquantitative results.