Lactoferrin ELISA 试剂盒
Quick Overview for Lactoferrin ELISA 试剂盒 (ABIN930713)
抗原
See all Lactoferrin (LTF) ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of Lactoferrin in the research laboratory
Alternative Names: Lactoferrin ELISA kit
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Lactotransferrin (LTF) ELISA Kit
LTF 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Milk, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Competition ELISA 3.12 μg/mL - 200 μg/mL Milk
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end-user
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板类型
- Pre-coated
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实验流程
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Highly purified Lactoferrin is bound to microwells . Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- Lactoferrin (LTF) (Lactotransferrin (LTF))
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别名
- Lactoferrin
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背景
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Lactoferrin (LF) is an iron-binding protein, which occurs in high concentrations in secretions at mucosa surfaces, in tears and in milk. LF also resides in the specific granules of polymorphnuclear neutrophil leukocytes (PMN) and becomes exocytosed upon PMN activation. During active inflammatory disease, raised serum levels of LF can be measured. The physiological anti microbial effect of Lactoferrin depends on its iron-binding capacity, because most bacteria require iron for their own physiological pathways. LF inhibits myelopoiesis, prevents complement activation and prevents the formation of hydroxl radicals. It is quite possible that LF has several important roles, like secretory IgA, as a nonspecific anti-phlogistic defence factor at mucosal surfaces. LF belongs to the pANCA, depending on the redistribuiton from the granules toward the nuclei, upon ethanol fixation. Autoantibodies against Lactoferrin occur in higher frequency in patients with rheumatoid vasculitis (RV), colitis ulcerosa (CU) and primary sclerosing cholangitis (PSC).
Synonyms: Lactoferrin ELISA kit. -
途径
- Transition Metal Ion Homeostasis
抗原 See all Lactoferrin (LTF) ELISA试剂盒
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