BPI ELISA 试剂盒
Quick Overview for BPI ELISA 试剂盒 (ABIN930709)
抗原
See all BPI ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of BPI in the research laboratory
Alternative Names: Bactericidal permeability-increasing protein ELISA kit, BPI ELISA kit
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Bactericidal/Permeability Increasing Protein (BPI) ELISA Kit
BPI 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Bactericidal/Permeability Increasing Protein (BPI) ELISA KitBPI 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum
Bactericidal/Permeability Increasing Protein (BPI) ELISA KitBPI 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
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应用备注
- Optimal conditions to be determined by end-user
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板类型
- Pre-coated
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实验流程
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Highly purified BPI is bound to microwells . Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- BPI (Bactericidal/Permeability Increasing Protein (BPI))
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别名
- anti-BPI
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物质类
- Antibody
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背景
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The main antigen for the cANCA is the proteinase 3 (PR3), which is a serine proteinase of the present in primary granules. Antibodies of pANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin G and also lysozyme often result in a similar pANCA pattern. Beside different untypical variants of pANCA IF patterns granulocyte specific antinuclear antibodies (GSANA) is indistinguishable from pANCA. This makes a clear interpretation and classification of the IF patterns difficult. Therefore every positive IFANCA findings especially pANCA should be differenciated by ELISA techniques using purified antigens. A survey of documented clinical indications of specific ANCA is given in the table below. PR3ANCA and MPOANCA are reliable serologic markers in the diagnostics of vasculitides. PR3ANCA is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95 %. cANCA is documented to be present in different diseases. The target antigen myeloperoxidase is mainly present (75 %) in microscopic polyangiitis.
Synonyms: Bactericidal permeability-increasing protein ELISA kit, BPI ELISA kit.
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