Anti-Rheumatoid Factor IgM ELISA 试剂盒
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- 抗原
- Anti-Rheumatoid Factor IgM
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
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ELISA kit for the detection of Rheumatoid Factor IgM in the research laboratory
Alternative Names: Rheumatoid Factor IgM ELISA kit
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- 应用备注
- Optimal conditions to be determined by end-user
- 板类型
- Pre-coated
- 实验流程
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Fc fragments of highly purified human Immunoglobulin G are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgM immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyses to form a blue colour.The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgM antibodies present in the original sample.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原
- Anti-Rheumatoid Factor IgM
- 物质类
- Antibody
- 背景
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The presence of IgM Rheumatoid Factor (RF) in the serum is the sole serological indicator included in the ACR list of criteria for the diagnosis of RA. RFs are a subset of antiglobulins directed against the Fc region of IgG. In this definition we do not include antibodies to the IgG Fab region and pepsin agglutinators, directed against neoantigens on IgG exposed by pepsin cleavage. Rheumatoid factors which have been found among the IgM, IgG and IgA classes of immunoglobulins, reacting only with xenogeneic Fc are not autoantibodies and are unlikely to be of pathological significance. However, RFs can bind IgG from many species, including autologous IgG, when immobilised on surfaces. Autologous binding is of higher affinity than xenogeneic binding. It is generally considered that high titers of RF are associated with more severe disease and the presence of extra-articular features and rheumatoid nodules.
Synonyms: Rheumatoid Factor IgM ELISA kit.
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