MYH11 ELISA 试剂盒
Quick Overview for MYH11 ELISA 试剂盒 (ABIN930691)
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检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of Sm1 in the research laboratory
Alternative Names: Smith antigen ELISA kit
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Myosin Heavy Chain 11, Smooth Muscle (MYH11) ELISA Kit
MYH11 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Myosin Heavy Chain 11, Smooth Muscle (MYH11) ELISA KitMYH11 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end-user
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板类型
- Pre-coated
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实验流程
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Highly purified RNP/Sm is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
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别名
- Sm1
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背景
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Today the best investigated immunoreactive antigens are double stranded DNA (dsDNA), single stranded DNA, snRNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SSA (Ro) and SSB (La). Smith Antigen (Sm) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE).
Synonyms: Smith antigen ELISA kit.
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